General Information

  • ID:  hor004553
  • Uniprot ID:  C0HKT6
  • Protein name:  RFamide precursor-related peptide 2
  • Gene name:  NA
  • Organism:  Agrotis ipsilon (Black cutworm moth)
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  Animal
  • Expression:  RFamide 1: Expressed in corpora cardiaca (CC), corpora allata (CA), antennal lobe (AL) and gnathal ganglion (GNG) (at protein level). Expression in AL detected in most animals, in CC, CA and in GNG in some animals (at protein level). RFamide precursor-rel
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Agrotis (genus), Noctuini (tribe), Noctuinae (subfamily), Noctuidae (family), Noctuoidea (superfamily), Obtectomera, Ditrysia, Heteroneura (parvorder), Neolepidoptera (infraorder), Glossata (suborder), Lepidoptera (order), Amphiesmenoptera (superorder), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  SYPPEPSAADIREAFERPT
  • Length:  19(48-66)
  • Propeptide:  MSCSRTVALLAALWLVVGATSSPVRRSPDLEARRRSAIDRSMIRFGRSYPPEPSAADIREAFERPTRRGNSFLRFGRSQPLTLSTDDLVSLLRAYEEDYDTPMTKKSASFVRFGRDPNFIRLGRSADDDKSAFEQNSELVVSGYPQRKSRARDHFIRLGRDSEEVNENEFEETEESRRKRSADSCHDCQS
  • Signal peptide:  MSCSRTVALLAALWLVVGATS
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  In insects, FMRFamide and related peptides have modulatory actions at skeletal neuromuscular junctions, and peptides that are immunologically related to FMRFamide are released into the circulation from neurohemal organs.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-C0HKT6-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004553_AF2.pdbhor004553_ESM.pdb

Physical Information

Mass: 245452 Formula: C94H141N25O32
Absent amino acids: CGHKLMNQVW Common amino acids: P
pI: 4.15 Basic residues: 2
Polar residues: 4 Hydrophobic residues: 5
Hydrophobicity: -106.84 Boman Index: -5517
Half-Life: 1.9 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 36.32
Instability Index: 6119.47 Extinction Coefficient cystines: 1490
Absorbance 280nm: 82.78

Literature

  • PubMed ID:  29466015
  • Title:  Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis Ipsilon Moths